| Congresso Brasileiro de Microbiologia 2023 | Resumo: 167-1 | ||||
Resumo:Non-typhoid Salmonella are one of the four main causes of foodborne bacterial infections worldwide. Salmonella enterica serovar Infantis (S. Infantis) is a foodborne and zoonotic pathogen of global distribution, capable to infect a broad range of food-producing animals besides humans. Despite of the leading role of Brazil in the global meat market, few studies employing genomic analyses have been conducted in this country in order to promote a better understanding of this serovar. Therefore, the aim of the present study was to characterize the genetic diversity of S. Infantis strains isolated from diverse sources in Brazil between 2014 and 2018 through comparative genomic analyses. A total of 80 S. Infantis strains isolated from veterinary sources (n=34), food items (n=27) and humans (n=19) in Brazil were analyzed by whole-genome multi-locus sequence typing (wgMLST) and compared to 18 representative genomes from Canada (n=2), Germany (n=3), Mexico (n=1), Peru (n=1), Turkey (n=1), United Kingdom (n=4) and the United States (n=6). The software Gegenees was employed to verify the genetic correlation of the 80 Brazilian genomes. Pan-genome was calculated and analyzed for three distinct subsets: for the 18 international genomes, for the 80 Brazilian genomes and for the total of 98 genomes analyzed. Prophages were searched in the 80 Brazilian genomes. A total of 25 Brazilian genomes were selected for the genome plasticity analysis using the software BLAST Ring Image Generator (BRIG), while five genomes were selected for the gene synteny analysis in the Mauve software. wgMLST allocated the 98 strains analyzed in five distinct clusters, being four mainly composed of Brazilian genomes and one exclusively by international genomes. Gegenees also revealed the presence of four distinct Brazilian clusters and an overall genomic similarity above 93%. The calculation of pan-genome’s α value revealed the presence of an open pan-genome in all three S. Infantis subsets analyzed. Fifteen types of prophages have been detected among the 80 strains from Brazil, being phage Fels 1 the most prevalent (48.75%). BRIG and Mauve demonstrated a high genomic structural conservation and a low occurrence of deletions and genetic arrangements among selected genomes. In conclusion, these results highlighted the high similarity among S. Infantis strains isolated in recent years and from diverse sources in Brazil, alerting for the potential hazard of its dissemination among food-producing animals, food items and humans in the country. Palavras-chave: comparative genomics, pangenome, Salmonella Infantis, wgMLST, whole-genome sequencing Agência de fomento:CAPES, CNPq, FAPESP | |||||